2/6/09 – Friday Observations
I observed the plates for the first time after a 48 hour incubation period. There was some barely observable growth, a colony or two on the MYPG plates and one of the MYPG w/CaCO3 plates. No observable smells yet other then the agar media. I left the plates to further incubate over the weekend.
2/9/09 – Monday Observations
Growth has bloomed on all the plates in five days. There is an obvious Brettanomyces funk smell to most of the plates. I’ve found the plates containing CaCO3 to be an unnecessary culture medium. There were fewer colony forming units (CFU’s), combined with slower overall growth on every CaCO3 plate compared with the standard MYPG plates. This could be a coincidence but I no longer feel it advantageous to use this medium. For further plating of cultures I will be using MYPG agar plates. The plates will be left in the incubator to further observe giant morphologies.
MYPG agar
This steak inoculation was done by
submersing the loop in the dregs
This plate was inoculated with
.1 ml of pipetted dregs
This plate was inoculated with
1 ml of pipetted dregs
MYPG w/CaCO3
This plate was inoculated with
.1 ml of pipetted dregs
This plate was inoculated with
.1 ml of pipetted dregs
After observing the plates I isolated 4 colonies from the various petri dishes. The single colonies were looped and transferred into 100 ml of YPD solution in a 250 ml Erlenmeyer flasks for propagation. A foam bung was inserted into the neck of the flask and the top was covered with a square piece of aluminum foil. These will be grown up at 28°C with constant shaking at 100 rpm in a Gallenkamp – Orbital Incubator. The same four colonies were also looped and streaked onto MYPG agar before being placed into the culturing chamber at 28°C. I will check back every 24 hours to observe the cell growth.