Fun with White Labs

Friday Lab Happenings

Finally got a chance to plate out the three White Labs vials which came in last week. As I need to make back-ups of all the Brettanomyces spp. which I collect in order to possibly re-propagate throughout the research, I inoculated two MYPG agar plates for each strain. The plates were inoculated with .1 ml of suspended yeast slurry and dispersed using glass beads. After inoculating, the plates were placed in the growth chamber at 28°C. Single colonies will then be transferred from the plates onto multiple MYPG agar slopes. MYPG agar has become my go to medium for storage and growth throughout the entire research project.

Pipetting out WhiteLabs B. claussenii
Pipetting out B. claussenii
(WLP 645)

WhiteLabs inoculated on MYPG agar
Done pipetting with a 1 liter starter
labeled and inoculated

WhiteLabs batch culture propagation
The three White Labs
Brett strains propagating
in the Orbital incubator

The remaining yeast suspensions were each poured into separate 1 liter starters. The 1.5 liter Erlenmeyer flasks were made up with 1 liter of MYPG solution without the addition of agar and autoclaved as stated in the MYPG recipes posted earlier. The flasks were then placed into the Gallenkamp – Orbital Incubator with constant shaking at 80 rpm and a temperature of 28°C.

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