Over the past couple of months I’ve been emailing with a graduate student at the University of California, Santa Cruz who was interested in running PCR identification on the two Brettanomyces strains (CMY001 and BSI-Drie) along with a few others. I just received an email detailing that PCR identification sequencing had been carried out on three of the Brettanomyces spp. used in this study and another that I have since acquired. It was determined that both the CMY001 and BSI-Drie strains are Brettanomyces bruxellensis and Wyeast 5151 is Brettanomyces anomalus. Thanks goes out to Michael Pearson for carrying out the PCR identification sequencing. The method used for identification was based on polymorphism in the rRNA Internal Transcribed Spacer Region. To read more about identifying Brettanomyces yeasts I’ve posted the methods used for the Identification of the “Drie” strain(s).
