Agar media recipes

WLN Agar Medium

A New Selective Media Agar Wallerstein Laboratory Nutrient (WLN) is a medium which is prepared already and sold by various lab companies. I used WLN from the Oxiod company with an additional 1% (1 g/l) of agar added to help further solidify the media for better streaking ability without gouging into the media. Here is […]

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Choosing Selective Agar Medias

Monday Lab Prep Made up three different media agars to promote the selective growth of Brettanomyces spp. and use for enumeration. I will test these out with the currently propagating cultures and save them for further use throughout the study to confirm my fermentations are uniquely Brettanomyces spp. The three media agars I chose to […]

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Lab Preparations

Day 1 in the Lab Made up two additions of MYPG agar in 500 ml volumes. One was with the addition of CaCO3 and the other without CaCO3. MYPG w/CaCO3 is similar to Dekkera media agar recommended for use in culturing Brettanomyces spp. by the European Brewery Convention (EBC). The calcium carbonate is supposed to […]

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