Yeast culturing
Wyeast to the Rescue
4/30/09 – Thursday Over the past month or so I have been e-mailing with Greg Doss of Wyeast Labs as previously mentioned and it turns out the gracious people over at Wyeast were able to supply the Brettanomyces Masters Project with three slopes. WY5112 (B. bruxellensis), WY5526 (B. lambicus) and WY5151 (B. claussenii) were shipped […]
Read moreMedia Cultureablity of Brettanomyces Species
Screening of Various Media Agars I have finished collecting and compiling data on the ability of B. bruxellensis (WLP650), B. lambicus (WLP653), B. claussenii (WLP645), B. bruxellensis (BSI-Drie) and B. bruxellensis (CMY001) to culture on popular brewery related media agar. This is the first part of the research and was carried out to find the […]
Read moreThe Many Faces of Brettanomyces
Giant Colony Morphologies When I undertook this project I never imagined the microbiology involved and the hours I would spend in the lab just to get this project off the ground. Three months in the lab culturing organisms so far and I have found that just working with Brettanomyces spp. on various media agars can […]
Read moreFun with White Labs
Friday Lab Happenings Finally got a chance to plate out the three White Labs vials which came in last week. As I need to make back-ups of all the Brettanomyces spp. which I collect in order to possibly re-propagate throughout the research, I inoculated two MYPG agar plates for each strain. The plates were inoculated […]
Read moreWLN Agar Medium
A New Selective Media Agar Wallerstein Laboratory Nutrient (WLN) is a medium which is prepared already and sold by various lab companies. I used WLN from the Oxiod company with an additional 1% (1 g/l) of agar added to help further solidify the media for better streaking ability without gouging into the media. Here is […]
Read moreOne Brett So Many Plates
Monday Observations I knew I had made up a lot of plates but when it came to critically observing each plate for growth and deciding how I should follow up with the results, it becomes time consuming and taking good notes is critical. It is counter balanced when observing the aroma of the plates though, […]
Read moreInoculation for Pure Culture Confirmation
2/17/09 – Tuesday Inoculation day Using the yeast slurry from each of the four flasks used to propagate up the Avery 15 Brettanomyces sp., I inoculated three different mediums (Copper Sulfate, MYPG+cycloheximide and Lysine agar) prepared the day earlier. First technique was to loop cells from the slurry and streak inoculate each of the different […]
Read morePropagation Observation
2/10/09 – Tuesday Propagation Observation There was no visible growth in the Brettanomyces propagation flasks yet as each flasks media solution was still unclouded. Further, there was no growth on the MYPG agar plates created from the single colony streaks. I will give it 48 hours and check back again on both. 2/12/09 – Thursday […]
Read moreAvery 15 Culture Plates
2/6/09 – Friday Observations I observed the plates for the first time after a 48 hour incubation period. There was some barely observable growth, a colony or two on the MYPG plates and one of the MYPG w/CaCO3 plates. No observable smells yet other then the agar media. I left the plates to further incubate […]
Read moreInoculating and Culturing the Dregs of Avery 15
Culturing Yeast From Avery’s 15th Anniversary Beer A few days prior to inoculating plates, I removed the special foil (and glue used to hold the foil) from the top and underside of an Avery 15 bottle cap by thoroughly cleaning and scrubbing with warm soapy water. After rinsing and drying the bottle, the neck of […]
Read more