Propagation

MYPG Compared to Wort as a Growth Substrate

While most of the batch culture propagations were conducted using wort as the growth substrate, I also looked at how batch culture growth proceeded using MYPG as the growth substrate. This study was performed with each of the three Brettanomyces strains from Wyeast by inoculating them  into 100 ml of MYPG substrate and 100 ml […]

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Propagation Data and Graphs

I have finished compiling the data on batch culture propagations conducted with five of the Brettanomyces strains. The following two graphs contain data from the standardized propagation method described in the previous post using 12 °Plato (1.048 gravity) wort as the growth medium. Some variation between growth patterns can be seen along with what appears […]

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Propagations Standardized

During the month of April and beginning of May, I worked to standardize batch culture propagation methods for maximizing cell growth. I had previously finished observing colony morphology and growth characteristics on various mediums and chose MYPG and WLN agar as my two mediums of choice. With eight strains of Brettanomyces to use throughout the […]

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Wyeast to the Rescue

4/30/09 – Thursday Over the past month or so I have been e-mailing with Greg Doss of Wyeast Labs as previously mentioned and it turns out the gracious people over at Wyeast were able to supply the Brettanomyces Masters Project with three slopes. WY5112 (B. bruxellensis), WY5526 (B. lambicus) and WY5151 (B. claussenii) were shipped […]

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Fun with White Labs

Friday Lab Happenings Finally got a chance to plate out the three White Labs vials which came in last week. As I need to make back-ups of all the Brettanomyces spp. which I collect in order to possibly re-propagate throughout the research, I inoculated two MYPG agar plates for each strain. The plates were inoculated […]

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Propagation Observation

2/10/09 – Tuesday Propagation Observation There was no visible growth in the Brettanomyces propagation flasks yet as each flasks media solution was still unclouded. Further, there was no growth on the MYPG agar plates created from the single colony streaks. I will give it 48 hours and check back again on both. 2/12/09 – Thursday […]

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